FAQs
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FAQs
Browse our frequently asked technical questions by selecting a product below.
Using crude blood as template reduces fluorescence, can I trust my results?
Yes, results are still accurate even with reduced fluorescence. We have observed even 10-fold reduction in the fluorescence plateau when amplifying the same target in crude blood versus extracted samples. However, there is no inhibition of the mix up to the levels we have tested (6.25% crude blood:EDTA), this can be verified either by normalising fluorescence between crude blood and other samples, or by modifying the threshold used so that it is always the same percentage of the max fluorescence reached (e.g., 10%).
